"Munc18-1 binds to syntaxin-1A via two distinct sites referred to as the ‘closed’ conformation and Nterminus binding. The latter has been shown to stimulate SNARE-mediated exocytosis, whereas the former is believed to be inhibitory or dispensable. To precisely define the contributions of each binding mode, we have engineered Munc18-1/-2 double knockdown neurosecretory cells and show that not only syntaxin-1A and -1B but also syntaxin-2 and -3 were significantly reduced as a result of Munc18-1 and - 2 knockdown."

File Number: MD-PC12 

Web site: http://www.uhnresearch.ca/tdc

Other Information: Publication: www.molbiolcell.org/content/early/2009/10/07/mbc.E09-08-0712.full.pdf+html